欧亚国际

最近科学家利用Plantarray功能表型生理系统，在知名期刊Horticulture Research发表题为“Understanding water conservation vs. profligation traits in vegetable legumes through a physio-transcriptomic-functional approach”的文章，这是今年发表的数篇文章之一。 

通过物理转录组学功能方法了解蔬菜豆类的保水性与抗旱性 

摘要 

蔬菜大豆和豇豆是相关的暖季豆类，在类似的根系干旱胁迫下表现出截然不同的叶片水分利用行为，其机制尚不清楚。在这里，我们对生长在反馈灌溉系统中的两种作物进行了综合表型转录组研究，该系统能够精确控制土壤含水量。连续蒸腾速率监测表明，豇豆在土壤干旱早期更保守地用水，但在长期干旱下往往保持较高的蒸腾速率。有趣的是，我们观察到在中度土壤干旱下，大豆的特定蒸腾速率增加，并伴随着相移。时间序列转录组学分析表明，豇豆在土壤干旱早期存在脱水回避机制，提示VuHAI3和VuTIP2基因参与其中。多因子基因聚类分析揭示了基因对干旱、一天中的时间以及它们在两种作物之间的相互作用的不同反应性，这涉及生物钟基因的物种依赖性调节。基因网络分析确定了两个共同表达模块，每个模块都与豇豆和大豆的蒸腾速率有关，包括物种之间的一对负相关模块。鉴定了模块枢纽基因，包括ABA降解基因GmCYP707A4和海藻糖磷酸酶/合酶基因VuTPS9。模块间网络分析揭示了中枢基因的推定共同参与者。转基因分析证实了VuTPS9在渗透胁迫下调节蒸腾速率的作用。这些发现表明，在遭受类似土壤干旱的两种作物的叶片中，物种特异性转录组重编程不仅是不同抗旱水平的结果，也是其原因之一。

Understanding water conservation vs. profligation traits in vegetable legumes through a physio-transcriptomic-functional approach 

Pingping Fang, Ting Sun, Arun Kumar Pandey, Libo Jiang, Xinyang Wu, Yannan Hu, Shiping Cheng, Mingxuan Li, Pei Xu

Horticulture Research, Volume 10, Issue 3, March 2023, uhac287, http://doi.org/10.1093/hr/uhac287

Abstract

Vegetable soybean and cowpea are related warm-season legumes showing contrasting leaf water use behaviors under similar root drought stresses, whose mechanisms are not well understood. Here we conducted an integrative phenomic-transcriptomic study on the two crops grown in a feedback irrigation system that enabled precise control of soil water contents. Continuous transpiration rate monitoring demonstrated that cowpea used water more conservatively under earlier soil drought stages, but tended to maintain higher transpiration under prolonged drought. Interestingly, we observed a soybean-specific transpiration rate increase accompanied by phase shift under moderate soil drought. Time-series transcriptomic analysis suggested a dehydration avoidance mechanism of cowpea at early soil drought stage, in which the VuHAI3 and VuTIP2;3 genes were suggested to be involved. Multifactorial gene clustering analysis revealed different responsiveness of genes to drought, time of day and their interactions between the two crops, which involved species-dependent regulation of the circadian clock genes. Gene network analysis identified two co-expression modules each associated with transpiration rate in cowpea and soybean, including a pair of negatively correlated modules between species. Module hub genes, including the ABA-degrading gene GmCYP707A4 and the trehalose-phosphatase/synthase gene VuTPS9 were identified. Inter-modular network analysis revealed putative co-players of the hub genes. Transgenic analyses verified the role of VuTPS9 in regulating transpiration rate under osmotic stresses. These findings propose that species-specific transcriptomic reprograming in leaves of the two crops suffering similar soil drought was not only a result of the different drought resistance level, but a cause of it.

2023年用Plantarray功能生理表型系统发表的部分文章

Plantarray是一款基于称重的高通量、多传感器生理表型平台以及植物逆境生物学研究通用平台。该系统可持续、实时测量位于不同环境条件下、阵列中每个植株的土壤-植物-空气（SPAC）中的即时水流动。直接测量根系和茎叶系统水平衡和生物量增加，计算植物生理参数以及植物对动态环境的反馈。系统以有效、易用、无损的方式针对植物对不同处理的反应、预测植物生长和生产力进行定量比较，广泛应用于生物胁迫和非生物胁迫以及植物栽培加速育种研究等，胁迫研究涵盖干旱胁迫、盐胁迫、重金属胁迫、热、冷胁迫、光胁迫以及灌溉/养分、CO2指示、植物健康等领域的研究。

1.Drought response behavior of risk-taking and conserving spring barley cultivars 

2.Drought and recovery in barley: key gene networks and retrotransposon response. 

3.Understanding water conservation vs. profligation traits in vegetable legumes through a physio-transcriptomic-functional approach 

4.Phenotypic plasticity in response to drought stress: Comparisons of domesticated tomato and a wild relative 

5.Tree tobacco (Nicotiana glauca) cuticular wax composition is essential for leaf retention during drought, facilitating a speedy recovery following rewatering 

6.Guard cell activity of PIF4 and HY5 control transpiration 

7.Functional physiological phenotyping and transcriptome analysis provide new insight into strawberry growth and water consumption