欧亚国际

SMO构建WIWAM XY高通量植物表型成像平台

WIWAM植物表型成像系统由比利时SMO公司与GHent大学VIB研究所研制生产，整合了LED植物只能培养、自动化控制系统、叶绿素荧光成像测量分析、植物热成像分析、植物近红外成像分析、植物高光谱分析、植物多光谱分析、植物CT断层扫描分析、自动条码识别管理、RGB真3D成像等多项先进技术，以优化的方式实现大量植物样品以优化的方式实现大量植物样品——从拟南芥、水稻、玉米到各种其它植物的生理生态与形态结构成像分析，用于高通量植物表型成像分析测量、植物胁迫响应成像分析测量、植物生长分析测量、生态毒理学研究、性状识别及植物生理生态分析研究等。

摘要

甲壳素已被证明是生长介质中矿物肥料的宝贵替代品。我们最近假设几丁质可能作为一种生物刺激剂，将促进植物生长微生物吸引到根际，特别是Mortierellales通过几丁质的应用大量增加，可能在几丁质介导的植物生长促进中发挥深远作用。我们从富含几丁质的生长培养基和生菜根际中分离出该目中的四个菌株，归类为Linnemannia elongata。通过多光谱成像测量，分离株在体外诱导拟南芥的地上部和根鲜重持续增加，叶绿素含量增加。通过研究分离物的基因组，我们假设这种生长促进是通过生长素的产生和/或Ca向植物的易位诱导的：（1）所有分离株的几丁质酶活性和几丁质降解潜力，以及（2）与不含几丁质的细长乳杆菌种子包衣相比，甲壳素和长乳杆菌种子包衣对拟南芥种子萌发的积极作用证明了甲壳素和长乳杆菌之间的协同作用。我们得出结论，与几丁质相关的生长促进取决于微生物群落的激活，其中长乳杆菌是关键物种。

Linnemannia elongata: a key species in chitin-based plant growth promotion

Abstract

Chitin has proven to be a valuable alternative for mineral fertilizers in growing media. We recently hypothesized that chitin might work as a biosestimulant, attracting plant growth promoting microbes to the rhizosphere. Especially Mortierellales increase massively in abundance by chitin application and might have a profound role in chitin-mediated plant growth promotion. We isolated four strains of this order, classified as Linnemannia elongata, from chitin-enriched growing medium and the lettuce rhizosphere. The isolates induced a consistent increase in shoot and root fresh weight and increased chlorophyll content of Arabidopsis thaliana in vitro as measured by multispectral imaging. By studying the isolate’s genomes, we postulate that this growth promotion is induced through auxin production and/or translocations of Ca to the plant. The synergy between chitin and L. elongata was demonstrated by (1) the chitinase activity and chitin degradation potential of all isolates and (2) the positive effect of chitin and L. elongata seed coating on germination of A. thaliana seeds as compared to L. elongata seed coating without chitin. We conclude that chitin-related growth promotion is depending on the activation of the microbial community, with L. elongata as a key species.