欧亚国际

来自比利时的科学家利用SMO搭建的高通量植物表型光谱无损成像平台，用以检测草莓疾病，该平台基于可见关与近红外（NIR）波长范围内特点吸收、反射和荧光模式，可实时对多种植物生理性状进行成像。表型平台的核心部分包括一台 3CCD 6 Mp—16 bit的相机，安装在直角坐标机器人上（SMO构建），相机配有12个光谱干涉滤波轮。

This multispectral imaging platform was used daily to record the lesion development of inoculated leaf discs floating in 24-well plates (24 replicates per light quality treatment and Botrytis isolate) from the day of inoculation until 4 days post-inoculation (dpi).

Phenotypic Variation of Botrytis cinerea Isolates Is Influenced by Spectral Light Quality

Lijuan Meng, Hanna Mestdagh, Maarten Ameye, Kris Audenaert, Monica Höfte and Marie-Christine Van Labeke*

Department of Plants and Crops, Faculty of Biosescience Engineering, Ghent University, Ghent, Belgium 

Botrytis cinerea, a fungal pathogen that causes gray mold, displays a high degree of phenotypic diversity. Light emitting diodes (LEDs) with specific light spectrum are increasingly used as lighting resource for plant greenhouse production. The chosen light spectrum can also have an effect on the pathogens in this production system. In this study, we investigated the phenological diversity in 15 B. cinerea isolates upon different light treatments. Daylight, darkness, and LED lights with different wavelengths (white, blue, red, blue+red) were chosen as treatments. The 15 Botrytis isolates differed in their mycelial growth rate, conidia production, and sclerotia formation. Light quality had a limited effect on growth rate. All isolates sporulated under daylight treatment, red light resulted in lower sporulation, while white, blue, and blue+red light inhibited sclerotia formation in all isolates, and sporulation in most, but not all isolates. Pathogenicity of the Botrytis isolates was studied on 2-week-old strawberry (Fragaria × ananassa ‘Elsanta’) leaves grown under white, blue, and red LED lights. The isolates differed in virulence on strawberry leaves, and this was positively correlated to oxalic acid production by B. cinerea in vitro. Red LED light improved leaf basal resistance to all the tested Botrytis isolates. Blue light pretreatment resulted in decreased leaf resistance to some isolates. Furthermore, we used image analysis to quantify the virulence of the different Botrytis isolates based on changes in photosynthetic performance of the strawberry leaves: chlorophyll fluorescence (Fv/Fm), chlorophyll index (ChlIdx) and anthocyanin content (modified anthocyanin reflection index, mAriIdx). Fv/Fm showed a strong negative correlation with disease severity and can be an indicator for the early detection of gray mold on strawberry leaves.