欧亚国际

WIWAM植物表型成像系统叶绿素荧光成像模块：基于叶绿素荧光成像的杂草机械和化学损伤胁迫监测 

目前，机械和化学损伤是进行杂草治理的主要方式。利用叶绿素荧光（CF）技术对杂草的胁迫生理状态进行无损监测，对揭示机械和化学胁迫以及复杂胁迫的破坏机制具有重要意义。在模拟的真实田间环境条件下，对不同品种和叶龄的杂草（洋地黄2-5叶龄，加拿大飞蓬5-10叶龄）进行1-7天的实验处理，并使用叶绿素荧光仪每24小时测量一次荧光参数。本研究的目的是研究不同种类杂草（洋地黄、加拿大飞蓬）在化学、机械和复合胁迫下不同胁迫位点的CF参数变化。结果表明，当杂草（Digitaria sanguinalis 和Erigeron canadensis)在不同部位受到化学胁迫，7天后它们的叶背部位受到的胁迫最为严重，光合抑制率达到R=75%。相反，机械应力与其变化不同，在一段时间的应力后，每个参数在1至2天后都会有所恢复，重机械应力R=11%。复合胁迫对CF参数的影响最为显著，主要表现在Fv/Fm、Fq'/Fm'、ETR、Rfd、NPQ和Y（NO）的变化时间和效率上，复合胁迫仅3-4天，R就达到71%-73%，复合胁迫的变化与其化学胁迫的变化模式基本一致。研究结果将有助于了解机械和化学应力以及复合应力对杂草CF参数的影响，并为未来有效的杂草控制操作和杂草控制提供指导。

关键字

杂草，机械应力，化学应力，复合应力，叶绿素

Monitoring weed mechanical and chemical damage stress based on chlorophyll fluorescence imaging

Currently, mechanical and chemical damage is the main way to carry out weed control. The use of chlorophyll fluorescence (CF) technology to nondestructively monitor the stress physiological state of weeds is significant to reveal the damage mechanism of mechanical and chemical stresses as well as complex stresses. Under simulated real field environmental conditions, different species and leaf age weeds (Digitaria sanguinalis 2-5 leaf age, and Erigeron canadensis 5-10 leaf age) were subjected to experimental treatments for 1-7 days, and fluorescence parameters were measured every 24 h using a chlorophyll fluorometer. The aim of this study was to investigate the changes in CF parameters of different species of weeds (Digitaria sanguinalis, Erigeron canadensis) at their different stress sites under chemical, mechanical and their combined stresses. The results showed that when weeds (Digitaria sanguinalis and Erigeron canadensis) were chemically stressed in different parts, their leaf back parts were the most severely stressed after 7 days, with photosynthetic inhibition reaching R=75%. In contrast, mechanical stress differs from its changes, and after a period of its stress, each parameter recovers somewhat after 1 to 2 days of stress, with heavy mechanical stress R=11%. Complex stress had the most significant effect on CF parameters, mainly in the timing and efficiency of changes in Fv/Fm, Fq’/Fm’, ETR, Rfd, NPQ and Y(NO), with R reaching 71%-73% after only 3-4 days of complex stress, and its changes in complex stress were basically consistent with the pattern of changes in its chemical stress. The results of the study will help to understand the effects of mechanical and chemical stresses and combined stresses on CF parameters of weeds and serve as a guide for efficient weed control operations and conducting weed control in the future. 

KEYWORDS

weeds, mechanical stress, chemical stress, complex stress, chlorophyll flfluorescence

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