欧亚国际

WIWAM植物表型成像系统叶绿素荧光成像模块：Barnase/Barstar在大豆中的引入为杂交育种提供了可挽救的雄性不育系统

摘要 

一个多世纪以来，在不需要更高投入的情况下，杂交育种一直被用于提高农业产量。虽然这种方法在作物生产力方面取得了一些最显著的进步，但育种障碍从根本上限制了大豆（Glycine max）从杂交活力中获益。大豆开出的花不显眼，在开放前会自我受精，因此不容易异交。在本研究中，我们证明了Barnase/Barstar雄性不育/雄性拯救系统可以用于大豆生产杂交种子。通过在大豆花药中绒毡层特异性启动子下表达细胞毒性核糖核酸酶Barnase，我们能够完全阻断花粉成熟，产生雄性不育植物。我们表明，当这些Barnase表达系与表达Barnase抑制剂Barstar的植物花粉杂交时，F1代的Barnase表达株系可以挽救生育能力。重要的是，我们发现成功挽救男性生育能力取决于Barnase和Barstar的相对剂量。当Barnase和Barstar在同一绒毡层特异性启动子下表达时，F1后代保持雄性不育。当我们在比Barnase相对更强的启动子下表达Barstar时，我们能够成功挽救F1一代的男性繁育能力。这项工作证明了生物技术方法在大豆中产生可育杂交后代的成功实施。

Introduction of Barnase/Barstar in soybean produces a rescuable male sterility system for hybrid breeding

Abstract

Hybrid breeding for increased vigor has been used for over a century to boost agricultural outputs without requiring higher inputs. While this approach has led to some of the most substantial gains in crop productivity, breeding barriers have fundamentally limited soybean ( Glycine max ) from reaping the benefits of hybrid vigor. Soybean makes inconspicuous flowers that self-fertilize before opening, and thus are not readily amenable to outcrossing. In this study, we demonstrate that the Barnase/Barstar male sterility/male rescue system can be used in soybean to produce hybrid seeds. By expressing the cytotoxic ribonuclease, Barnase, under a tapetum-specific promoter in soybean anthers, we are able to completely block pollen maturation, creating male-sterile plants. We show that fertility can be rescued in the F1 generation of these Barnase-expressing lines when they are crossed with pollen from plants that express the Barnase inhibitor, Barstar. Importantly, we found that the successful rescue of male fertility is dependent on the relative dosage of Barnase and Barstar. When Barnase and Barstar were expressed under the same tapetum-specific promoter, the F1 offspring remained male-sterile. When we expressed Barstar under a relatively stronger promoter than Barnase, we were able to achieve a successful rescue of male fertility in the F1 generation. This work demonstrates the successful implementation of a biotechnology approach to produce fertile hybrid offspring in soybean.

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